Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I2 R1	52	300.6	3297834	97.8%	3225281	57.7

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	432,019	+A	coding (385/1407 nt)	ybfM →	predicted outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	432,015	1	.	A	100.0%	103.4 / NA	32	D127E (GAC→GAA)	ybfM	predicted outer membrane porin
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (32/0); total (32/0)

CCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑AAAAGCGGTATAAGCCTGTA > minE/431955‑432035

cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:956064/62‑1 (MQ=255)

cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:1088980/62‑1 (MQ=255)

cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:1534815/62‑1 (MQ=255)

cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:2271977/62‑1 (MQ=255)

cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:2084194/62‑1 (MQ=255)

cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:1908761/62‑1 (MQ=255)

        aaaTCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:1922518/54‑1 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAGGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2615754/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:308518/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2693297/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2802995/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2843418/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2947558/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2960996/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2513436/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:366092/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:391785/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:681878/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:811675/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:84908/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:896063/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:911324/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:96653/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1776051/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1192414/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1199270/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1260665/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1481744/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1612097/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1632970/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1707706/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2652063/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1853871/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2248656/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2254497/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2350492/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2423465/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2566802/1‑60 (MQ=255)

                      aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTCta  >  1:2921329/1‑60 (MQ=255)

                          gTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:1599508/36‑1 (MQ=255)

CCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑AAAAGCGGTATAAGCCTGTA > minE/431955‑432035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A