Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I3 R1
|
26 |
437.0 |
1038814 |
65.5% |
680423 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,497,293 |
G→A |
N83N (AAC→AAT) |
nuoF ← |
NADH:ubiquinone oxidoreductase, chain F |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,497,293 | 0 | G | A | 100.0%
| 11.5
/ NA
| 5 | N83N (AAC→AAT) | nuoF | NADH:ubiquinone oxidoreductase, chain F |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (5/0); total (5/0) |
TATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCG > minE/1497244‑1497305
|
tataGGTGCCCGGCTCCATTTCATCGGCGTTACACAGTAGGTAACGGATATTCATGGATTCg > 1:4189/1‑62 (MQ=255)
tataGGTGCCCGGCTCCATTTCATCGGCGTTACACAGTAGGTAACGGATATTCATGGATTCg > 1:6572/1‑62 (MQ=255)
tataGGTGCCCGGCTCCATTTCATCGGCGTTACACAGTAGGTAACGGATATTCATGGATTCg > 1:728004/1‑62 (MQ=255)
tataGGTGCCCGGCTCCATTTCATCGGCGTTACACAGTAGGTAACGGATATTCATGGATTCg > 1:768639/1‑62 (MQ=255)
tataGGTGCCCGGCTCCATTTCATCGGCGTTACACAGTAGGTAACGGATATTCATGGATTCg > 1:774053/1‑62 (MQ=255)
|
TATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCG > minE/1497244‑1497305
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
