Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I3 R1
|
26 |
437.0 |
1038814 |
65.5% |
680423 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,967,244 |
G→A |
D462D (GAC→GAT) |
tktA ← |
transketolase 1, thiamin‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,967,244 | 0 | G | A | 100.0%
| 14.2
/ NA
| 10 | D462D (GAC→GAT) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/10); total (0/10) |
GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT > minE/1967193‑1967254
|
gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga < 1:1003303/62‑2 (MQ=12)
gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga < 1:1030947/62‑2 (MQ=12)
gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga < 1:33402/62‑2 (MQ=12)
gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga < 1:480844/62‑2 (MQ=12)
gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga < 1:620481/62‑2 (MQ=12)
gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga < 1:661560/62‑2 (MQ=12)
gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga < 1:894375/62‑2 (MQ=12)
gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga < 1:924550/62‑2 (MQ=12)
gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCACAGGCCGATCGAATCGTGGgtga < 1:948588/62‑2 (MQ=255)
cGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga < 1:859212/38‑2 (MQ=17)
|
GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT > minE/1967193‑1967254
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
