Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I3 R1
|
26 |
437.0 |
1038814 |
65.5% |
680423 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,111,795 |
T→C |
C147R (TGC→CGC) |
agaI → |
galactosamine‑6‑phosphate isomerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,111,795 | 0 | T | C | 100.0%
| 24.9
/ NA
| 8 | C147R (TGC→CGC) | agaI | galactosamine‑6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/8); total (0/8) |
AACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCA > minE/2111762‑2111823
|
aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa < 1:122925/62‑1 (MQ=255)
aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa < 1:272610/62‑1 (MQ=255)
aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa < 1:44227/62‑1 (MQ=255)
aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa < 1:538380/62‑1 (MQ=255)
aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa < 1:590260/62‑1 (MQ=255)
aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa < 1:852461/62‑1 (MQ=255)
aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa < 1:920292/62‑1 (MQ=255)
aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGGCa < 1:696942/62‑1 (MQ=255)
|
AACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCA > minE/2111762‑2111823
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
