Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,213,568 |
C→T |
intergenic (+24/+55) |
rpoD → / ← ygjF |
RNA polymerase, sigma 70 (sigma D) factor/G/U mismatch‑specific DNA glycosylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,213,568 | 0 | C | T | 100.0%
| 119.5
/ NA
| 38 | intergenic (+24/+55) | rpoD/ygjF | RNA polymerase, sigma 70 (sigma D) factor/G/U mismatch‑specific DNA glycosylase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/25); total (13/25) |
TTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTA > W3110S.gb/3213511‑3213625
|
ttCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTAtgccg < 1:3312876/62‑1 (MQ=255)
ttCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTAtgccg < 1:4226174/62‑1 (MQ=255)
ttCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTAtgccg < 1:560584/62‑1 (MQ=255)
ttCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTAtgccg < 1:2047181/62‑1 (MQ=255)
ttCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTAtgccg < 1:2140921/62‑1 (MQ=255)
gAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCAc < 1:1189468/61‑1 (MQ=255)
gAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCAc < 1:46467/61‑1 (MQ=255)
gAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCAc < 1:2945951/61‑1 (MQ=255)
gctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGc > 1:3761069/1‑61 (MQ=255)
gctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGc > 1:3911021/1‑61 (MQ=255)
gctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGc > 1:3180580/1‑61 (MQ=255)
gctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGc > 1:1325330/1‑61 (MQ=255)
gctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGc > 1:1481834/1‑61 (MQ=255)
gctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCa < 1:249372/62‑1 (MQ=255)
gctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCa < 1:1625927/62‑1 (MQ=255)
gctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCa < 1:4439987/62‑1 (MQ=255)
gctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACACgg > 1:4240652/1‑60 (MQ=38)
gctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACACGGc > 1:259078/1‑61 (MQ=255)
ctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCAc < 1:1935668/55‑1 (MQ=255)
ctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCa < 1:1158424/61‑1 (MQ=255)
tCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCctc > 1:502709/1‑61 (MQ=255)
tCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCctc > 1:2828567/1‑61 (MQ=255)
ggACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTg > 1:67766/1‑59 (MQ=255)
ggACGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGca > 1:40750/1‑61 (MQ=255)
aCGATTAATCGGTAGGCCGGATCAGGCGTTATGCCGCAc < 1:1950646/39‑1 (MQ=255)
aTTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACg < 1:545823/62‑1 (MQ=255)
aTTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACg < 1:3834263/62‑1 (MQ=255)
aTTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACg < 1:914739/62‑1 (MQ=255)
aTTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACg < 1:2770716/62‑1 (MQ=255)
aTTAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACg < 1:1364007/62‑1 (MQ=255)
ttAATCGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACg < 1:2220265/61‑1 (MQ=255)
cGGTAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACg < 1:3462318/56‑1 (MQ=255)
tAGGCCGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCtttt > 1:306127/1‑62 (MQ=255)
ccGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTctg < 1:4500089/61‑3 (MQ=255)
ccGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCgg < 1:3068519/61‑1 (MQ=255)
ccGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCgg < 1:4553698/61‑1 (MQ=255)
ccGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCgg < 1:1911204/61‑1 (MQ=255)
ccGGATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTCCACAAACGCCACCTTTTCgg < 1:3135798/61‑1 (MQ=39)
ggATCAGGCGGTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTgg < 1:3051730/62‑1 (MQ=255)
gATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGc < 1:3747588/62‑1 (MQ=255)
gATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGc < 1:3559733/62‑1 (MQ=255)
gATCAGGCGTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGc < 1:2912867/62‑1 (MQ=255)
gTTATGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTa > 1:4143562/1‑62 (MQ=255)
|
TTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTA > W3110S.gb/3213511‑3213625
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
