Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,532,412 |
T→C |
M236T (ATG→ACG) |
cpxA → |
sensory histidine kinase in two‑component regulatory system with CpxR |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,532,412 | 0 | T | C | 100.0%
| 86.5
/ NA
| 24 | M236T (ATG→ACG) | cpxA | sensory histidine kinase in two‑component regulatory system with CpxR |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/12); total (12/12) |
GCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACG > W3110S.gb/3532372‑3532470
|
gCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCtctc < 1:3093652/49‑1 (MQ=255)
gCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCtctc < 1:754184/49‑1 (MQ=255)
gCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCtctc < 1:518136/49‑1 (MQ=255)
gCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCtctc < 1:3746041/49‑1 (MQ=255)
gCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCtctc < 1:2387768/49‑1 (MQ=255)
gCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCtctc < 1:2318784/49‑1 (MQ=255)
ttttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCt > 1:4460779/1‑61 (MQ=255)
ttttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCt > 1:4453590/1‑61 (MQ=255)
ttttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCt > 1:4313044/1‑61 (MQ=255)
ttttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCt > 1:3841561/1‑61 (MQ=255)
ttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGat > 1:2735347/1‑62 (MQ=255)
ttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGat > 1:3467113/1‑62 (MQ=255)
ttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGat > 1:3267594/1‑62 (MQ=255)
ttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGa > 1:2634711/1‑61 (MQ=255)
ccAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATAtc < 1:1535371/61‑1 (MQ=255)
ccAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATAtc < 1:3090570/61‑1 (MQ=255)
ccAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATAtc < 1:2138176/61‑1 (MQ=255)
ccAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATAtc < 1:2336195/61‑1 (MQ=255)
gATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATAtctctc > 1:1472572/1‑62 (MQ=255)
gATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATAtctctc > 1:4283710/1‑62 (MQ=255)
gATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATAtctctc > 1:2575249/1‑62 (MQ=255)
tCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAg > 1:1994629/1‑62 (MQ=255)
gcgcATGACGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCg < 1:2672764/61‑1 (MQ=255)
gcgcATGACGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCg < 1:1593697/61‑1 (MQ=255)
tgacgaCCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGAcg < 1:4101883/62‑1 (MQ=255)
tgacgaCCTCTCAGCAGCGTCTGCTTTCTGATATCTATCACGAGCTGCGCACCCCGCTGAcg < 1:681947/62‑1 (MQ=255)
|
GCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACG > W3110S.gb/3532372‑3532470
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
