Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,639,730 |
T→C |
K222R (AAA→AGA) |
xerC ← |
site‑specific tyrosine recombinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,639,730 | 0 | T | C | 100.0%
| 133.3
/ NA
| 36 | K222R (AAA→AGA) | xerC | site‑specific tyrosine recombinase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/2); total (34/2) |
ATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGT > W3110S.gb/3639681‑3639787
|
aTGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTAc < 1:2173870/62‑1 (MQ=255)
aTGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTAc < 1:3141092/62‑1 (MQ=255)
aGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCtt > 1:322295/1‑62 (MQ=255)
ccTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGga > 1:967612/1‑50 (MQ=255)
ccTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGga > 1:159458/1‑50 (MQ=255)
ccTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTg > 1:3487431/1‑60 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:395976/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:387426/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:1177548/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:3297307/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:4086025/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:320006/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:4430508/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:4535591/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:279937/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:677524/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:768370/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:2432341/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgcg > 1:1843476/1‑38 (MQ=255)
tGCCCCATTCGGCAAAGCGTCTCTGCACATTAcgcgc > 1:2438481/1‑37 (MQ=255)
aaGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGt > 1:3567063/1‑42 (MQ=255)
aaGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc > 1:4017828/1‑62 (MQ=255)
aaGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc > 1:4133888/1‑62 (MQ=255)
aaGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc > 1:4554426/1‑62 (MQ=255)
aaGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc > 1:48729/1‑62 (MQ=255)
aaGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc > 1:2678087/1‑62 (MQ=255)
aaGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc > 1:1400161/1‑62 (MQ=255)
aaGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgt > 1:3212362/1‑61 (MQ=255)
aaGCGTCCCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc > 1:3069797/1‑62 (MQ=255)
gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:4157470/1‑62 (MQ=255)
gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:4203471/1‑62 (MQ=255)
gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:2828594/1‑62 (MQ=255)
gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:611311/1‑62 (MQ=255)
gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:661869/1‑62 (MQ=255)
gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:2718474/1‑62 (MQ=255)
gCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:836260/1‑62 (MQ=255)
|
ATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGT > W3110S.gb/3639681‑3639787
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
