Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,704,876 |
C→T |
M164I (ATG→ATA) |
trkD ← |
potassium transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,704,876 | 0 | C | T | 100.0%
| 150.5
/ NA
| 45 | M164I (ATG→ATA) | trkD | potassium transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/21); total (24/21) |
CCAGTCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTGAGAGG > W3110S.gb/3704818‑3704933
|
ccAGTCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACtata < 1:3603519/62‑1 (MQ=255)
ccAGTCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACtata < 1:2821159/62‑1 (MQ=255)
cAGTCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACtata < 1:2128051/61‑1 (MQ=255)
tCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCg > 1:106118/1‑62 (MQ=255)
tCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCg > 1:4695808/1‑62 (MQ=255)
tCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCg > 1:3506724/1‑62 (MQ=255)
tCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCg > 1:3040420/1‑62 (MQ=255)
tCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCg > 1:2836618/1‑62 (MQ=255)
tCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCg > 1:2542647/1‑62 (MQ=255)
tCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCg > 1:197263/1‑62 (MQ=255)
tCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCg > 1:1395076/1‑62 (MQ=255)
tCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCg > 1:1407061/1‑62 (MQ=255)
gAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGt > 1:1131102/1‑62 (MQ=255)
gAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGt > 1:3018132/1‑62 (MQ=255)
gAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGt > 1:1303920/1‑62 (MQ=255)
aGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCata < 1:894156/62‑1 (MQ=255)
aGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCata < 1:1207577/62‑1 (MQ=255)
aGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCata < 1:1920743/62‑1 (MQ=255)
aGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCata < 1:1852073/62‑1 (MQ=255)
aTCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATaaa > 1:75294/1‑60 (MQ=255)
aTCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATaaa > 1:2043608/1‑60 (MQ=255)
aTCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATaaa > 1:3576430/1‑60 (MQ=255)
aTCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATaaa > 1:3539223/1‑60 (MQ=255)
aTCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATaaa > 1:3200040/1‑60 (MQ=255)
aTCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATaaa > 1:310557/1‑60 (MQ=255)
aTCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATaaa > 1:3088063/1‑60 (MQ=255)
cgcAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTgaga < 1:1077851/61‑1 (MQ=255)
cgcAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTgaga < 1:1447092/61‑1 (MQ=255)
cgcAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTgaga < 1:3162552/61‑1 (MQ=255)
cgcAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTgaga < 1:2970833/61‑1 (MQ=255)
gcAAACAGCTTACCGACTATAGCCGTGACATGTTTTTGAATCATAAATAATAAAGTgaga < 1:1886640/60‑1 (MQ=255)
cAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAAc > 1:199346/1‑61 (MQ=255)
cAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAAc > 1:2048844/1‑61 (MQ=255)
cAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAAc > 1:1337692/1‑61 (MQ=255)
cAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACg < 1:1060202/62‑1 (MQ=255)
cAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACg < 1:3912001/62‑1 (MQ=255)
aaaCAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACg < 1:1578005/61‑1 (MQ=255)
aCAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATa > 1:1224947/1‑62 (MQ=255)
cTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTg < 1:1676439/62‑1 (MQ=255)
cTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTg < 1:4459617/62‑1 (MQ=255)
ttACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTg < 1:2677934/61‑1 (MQ=255)
ccGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATa > 1:646660/1‑54 (MQ=255)
gACTATAGCCGTGCCATGTTTTTGAATCTTAAATAATAAAGTGAGAACGATAATTGAGAgg < 1:2204744/61‑1 (MQ=255)
gACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTTAGAACGATAATTGAGAgg < 1:6761/61‑1 (MQ=255)
gACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTGAGAgg < 1:4422442/61‑1 (MQ=255)
gACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTGAGAgg < 1:4432663/61‑1 (MQ=255)
gACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTGAGAgg < 1:1540982/61‑1 (MQ=255)
gACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTGAGAgg < 1:1410565/61‑1 (MQ=255)
|
CCAGTCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTGAGAGG > W3110S.gb/3704818‑3704933
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
