Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,588,402 |
C→T |
L1032L (CTG→CTA) |
hsdR ← |
endonuclease R |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,588,402 | 0 | C | T | 100.0%
| 176.9
/ NA
| 53 | L1032L (CTG→CTA) | hsdR | endonuclease R |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (34/19); total (34/19) |
GCGGGCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGC > W3110S.gb/4588347‑4588456
|
gcgGGCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGa > 1:1302791/1‑61 (MQ=255)
gcgGGCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGa > 1:620914/1‑61 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:343186/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:2016311/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:3034663/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:1724847/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:2064039/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:2620882/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:3315556/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:161794/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:1554690/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:543701/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:1000075/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:3764333/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:4072921/1‑62 (MQ=255)
gCGATTAATAACTGCCTGAAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa > 1:2016974/1‑62 (MQ=255)
aTTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAgg > 1:1706262/1‑62 (MQ=255)
aTTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAgg > 1:2115700/1‑62 (MQ=255)
aTTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAgg > 1:3042326/1‑62 (MQ=255)
aTTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAgg > 1:1701067/1‑62 (MQ=255)
aTTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAgg > 1:1357840/1‑62 (MQ=255)
taaCTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTc < 1:2112380/61‑1 (MQ=255)
taaCTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTc < 1:511592/61‑1 (MQ=255)
taaCTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTc < 1:805050/61‑1 (MQ=255)
taaCTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTc < 1:2353969/61‑1 (MQ=255)
taaCTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTc < 1:4215492/61‑1 (MQ=255)
taaCTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTc < 1:1732233/61‑1 (MQ=255)
aaCTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTc < 1:3765602/60‑1 (MQ=255)
aCTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTc < 1:2716124/59‑1 (MQ=255)
tgcctgcAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGa > 1:2889842/1‑62 (MQ=255)
tgcctgcAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGa > 1:1731238/1‑62 (MQ=255)
cAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAATGGCTTCGAGGAAATCCt > 1:424956/1‑62 (MQ=255)
cAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCt > 1:2183537/1‑62 (MQ=255)
cAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCt > 1:4186446/1‑62 (MQ=255)
cAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCt > 1:1235144/1‑62 (MQ=255)
cAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCt > 1:1548336/1‑62 (MQ=255)
ccGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTgcggc < 1:4618418/62‑1 (MQ=255)
ccGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTgcggc < 1:3782654/62‑1 (MQ=255)
ccGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTgcggc < 1:1285352/62‑1 (MQ=255)
ccGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTgcggc < 1:1638577/62‑1 (MQ=255)
ccGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTgcggc < 1:1718015/62‑1 (MQ=255)
gcgcGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTc > 1:4709085/1‑61 (MQ=255)
gcgcGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTc > 1:4049935/1‑61 (MQ=255)
gcgcGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTc > 1:873276/1‑61 (MQ=255)
gcgcGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTc > 1:1625071/1‑61 (MQ=255)
gcgcGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTc > 1:1082372/1‑61 (MQ=255)
gAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTgcggc < 1:2614523/45‑1 (MQ=255)
cGTTGCACTAGCCAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACa > 1:348817/1‑61 (MQ=255)
gTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACa < 1:3186668/60‑1 (MQ=255)
gTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGc < 1:3819296/62‑1 (MQ=255)
gTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGc < 1:2527573/62‑1 (MQ=255)
gTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGc < 1:873410/62‑1 (MQ=255)
gTTGCACTAGCGAGTCAAAGACTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGc < 1:2093568/62‑1 (MQ=255)
|
GCGGGCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGC > W3110S.gb/4588347‑4588456
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
