Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,591,445 |
G→A |
T18I (ACA→ATA) |
hsdR ← |
endonuclease R |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,591,445 | 0 | G | A | 100.0%
| 127.1
/ NA
| 40 | T18I (ACA→ATA) | hsdR | endonuclease R |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (35/5); total (35/5) |
ATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAACATCTCAT > W3110S.gb/4591385‑4591502
|
aTGGCATAAGTGAAGTCGTT‑ACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCattatt < 1:4176526/62‑1 (MQ=255)
tGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCattatt < 1:3957021/62‑1 (MQ=255)
tGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCattatt < 1:3739371/62‑1 (MQ=255)
tGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCattatt < 1:3982433/62‑1 (MQ=255)
gCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCAttatta > 1:266799/1‑61 (MQ=255)
gCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATtattat < 1:3272973/62‑1 (MQ=255)
aaGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAAt < 1:3093892/62‑1 (MQ=255)
tCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCt > 1:4636364/1‑62 (MQ=255)
tCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCt > 1:3738675/1‑62 (MQ=255)
tCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCt > 1:3138806/1‑62 (MQ=255)
tCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCt > 1:4320449/1‑62 (MQ=255)
tCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCt > 1:4364451/1‑62 (MQ=255)
tCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCt > 1:1358852/1‑62 (MQ=255)
tGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCattatt < 1:4132973/44‑1 (MQ=255)
gCCCTTCAGGAATTCAAAATTGGATTTATTCATCattatt < 1:4224490/40‑1 (MQ=255)
gCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGc > 1:3974059/1‑62 (MQ=255)
cccTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGc > 1:3934748/1‑61 (MQ=255)
cccTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGc > 1:2045498/1‑61 (MQ=255)
cccTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGc > 1:261385/1‑61 (MQ=255)
cccTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGc > 1:1290038/1‑61 (MQ=255)
cccTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGc > 1:2188214/1‑61 (MQ=255)
cccTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGc > 1:850069/1‑61 (MQ=255)
cccTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGc > 1:660760/1‑61 (MQ=255)
cccTTCAGGAATTCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGc > 1:3761698/1‑61 (MQ=255)
tCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAatat > 1:1544865/1‑61 (MQ=255)
tCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAatat > 1:734934/1‑61 (MQ=255)
tCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAatat > 1:2101079/1‑61 (MQ=255)
tCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAATAtt < 1:1036539/62‑1 (MQ=255)
tCAAAATTGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAATAtt < 1:4500016/62‑1 (MQ=255)
ttGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGc < 1:2746680/62‑1 (MQ=255)
ttGGATTTATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGc < 1:4585178/62‑1 (MQ=255)
tATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTg > 1:4490314/1‑39 (MQ=255)
tATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTg > 1:621932/1‑39 (MQ=255)
tATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTg > 1:3057690/1‑39 (MQ=255)
tATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTg > 1:3009842/1‑39 (MQ=255)
tATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTg > 1:821665/1‑39 (MQ=255)
tATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTg > 1:2397557/1‑39 (MQ=255)
tATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTg > 1:2386647/1‑39 (MQ=255)
tATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTg > 1:2355382/1‑39 (MQ=255)
tATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTg > 1:1604672/1‑39 (MQ=255)
tATTCATCATTATTATTAATCCATTGCTGTGCGGGCCTg > 1:983543/1‑39 (MQ=255)
ttCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAAcat > 1:769987/1‑62 (MQ=255)
ttCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAAcat > 1:1184092/1‑62 (MQ=255)
ttCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAAcat > 1:3063871/1‑62 (MQ=255)
ttCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAAcat > 1:1395305/1‑62 (MQ=255)
ttCATCATTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAAca > 1:2415407/1‑61 (MQ=255)
ttCATCAGTATTATTAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAAcat > 1:1423890/1‑62 (MQ=255)
tcatTATTATTAATCCATTGCTGTTCGGGCCTGTCCAAATATTTAAGGCCCATAAcatctca > 1:3823377/1‑62 (MQ=255)
attattattAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAAcatctcat > 1:4387804/1‑61 (MQ=255)
attattattAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAAcatctcat > 1:3890233/1‑61 (MQ=255)
attattattAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAAcatctcat > 1:355911/1‑61 (MQ=255)
attattattAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAAcatctcat > 1:2742300/1‑61 (MQ=255)
attattattAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAAcatctcat > 1:2426917/1‑61 (MQ=255)
|
ATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCTGTGCGGGCCTGTCCAAATATTTAAGGCCCATAACATCTCAT > W3110S.gb/4591385‑4591502
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
