Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
455,022 |
C→T |
H222H (CAC→CAT) |
tig → |
peptidyl‑prolyl cis/trans isomerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 455,022 | 0 | C | T | 85.7%
| 48.1
/ 1.7
| 21 | H222H (CAC→CAT) | tig | peptidyl‑prolyl cis/trans isomerase |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (0/18); total (1/20) |
| Fisher's exact test for biased strand distribution p-value = 1.43e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAA > W3110S.gb/454975‑455079
|
gCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGaa > 1:2700391/1‑62 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAGGGTAAAGCAGCGAAATTCGCTATCaa < 1:2743038/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:2564402/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:801964/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:768241/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:720161/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:608657/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:477047/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:3033666/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:2965721/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:2582843/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1020839/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:2496711/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:2284353/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1834038/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1670020/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1658721/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1414128/62‑1 (MQ=255)
aCCTTCCCGGAAGAATACCATGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCaa < 1:1224158/62‑1 (MQ=255)
aCCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGaa < 1:2036979/62‑1 (MQ=255)
aCCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGaa < 1:1538485/62‑1 (MQ=255)
|
GCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAA > W3110S.gb/454975‑455079
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
