Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
726,557 |
A→G |
V301A (GTT→GCT) |
kdpB ← |
potassium translocating ATPase, subunit B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 726,557 | 0 | A | G | 100.0%
| 30.9
/ NA
| 10 | V301A (GTT→GCT) | kdpB | potassium translocating ATPase, subunit B |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (1/9); total (1/9) |
TGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACG > W3110S.gb/726520‑726582
|
tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc < 1:1002254/62‑1 (MQ=255)
tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc < 1:1108801/62‑1 (MQ=255)
tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc < 1:1866395/62‑1 (MQ=255)
tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc < 1:2507980/62‑1 (MQ=255)
tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc < 1:2926589/62‑1 (MQ=255)
tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc < 1:2935633/62‑1 (MQ=255)
tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc < 1:2972124/62‑1 (MQ=255)
tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc < 1:810767/62‑1 (MQ=255)
gtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc < 1:251559/61‑1 (MQ=255)
gtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCACg > 1:247074/1‑62 (MQ=255)
|
TGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACG > W3110S.gb/726520‑726582
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
