Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
770,570 |
+G |
coding (2171/2634 nt) |
mngB → |
alpha‑mannosidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 770,567 | 1 | . | G | 88.9%
| 23.3
/ ‑2.6
| 9 | coding (2168/2634 nt) | mngB | alpha‑mannosidase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (0/8); total (0/9) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 |
GGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTC‑GGGAATTAAAATGCCAGTCCCGG > W3110S.gb/770521‑770590
|
ggCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTC‑GGGAATTAAAATGcc < 1:1619420/62‑1 (MQ=255)
agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:1090109/54‑1 (MQ=255)
agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:1091931/54‑1 (MQ=255)
agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:1144827/54‑1 (MQ=255)
agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:1987941/54‑1 (MQ=255)
agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:2420853/54‑1 (MQ=255)
agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:2432382/54‑1 (MQ=255)
agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:2797325/54‑1 (MQ=255)
agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:668066/54‑1 (MQ=255)
|
GGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTC‑GGGAATTAAAATGCCAGTCCCGG > W3110S.gb/770521‑770590
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
