Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
776,135 |
G→C |
P187P (CCG→CCC) |
tolQ → |
membrane spanning protein in TolA‑TolQ‑TolR complex |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 776,135 | 0 | G | C | 100.0%
| 12.2
/ NA
| 5 | P187P (CCG→CCC) | tolQ | membrane spanning protein in TolA‑TolQ‑TolR complex |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (5/0); total (5/0) |
CCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGT > W3110S.gb/776080‑776140
|
cgggTATTGCCGAAGCCCTGATCGCCACCGCGATCGGCCTGTTCGCCGCTATCCCCGCAGt > 1:2309689/3‑61 (MQ=255)
cgggTATTGCCGAAGCCCTGATCGCCACCGCGATCGGCCTGTTCGCCGCTATCCCCGCAGt > 1:2825727/3‑61 (MQ=255)
cgggTATTGCCGAAGCCCTGATCGCCACCGCGATCGGCCTGTTCGCCGCTATCCCCGCAGt > 1:2884175/3‑61 (MQ=255)
cgggTATTGCCGAAGCCCTGATCGCCACCGCGATCGGCCTGTTCGCCGCTATCCCCGCAGt > 1:437743/3‑61 (MQ=255)
cgggTATTGCCGAAGCCCTGATCGCCACCGAGATCGGCCTGTTCGCCGCTATCCCCGCAGt > 1:1781066/3‑61 (MQ=255)
|
CCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGT > W3110S.gb/776080‑776140
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
