Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
875,632 |
C→T |
S744S (AGC→AGT) |
yliE → |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 875,632 | 0 | C | T | 97.9%
| 145.2
/ ‑6.2
| 47 | S744S (AGC→AGT) | yliE | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (16/30); minor base G (0/1); total (16/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATAACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAG > W3110S.gb/875595‑875680
|
ataACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATc < 1:338684/62‑1 (MQ=255)
ataACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATc < 1:2713828/62‑1 (MQ=255)
ataACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATc < 1:2486902/62‑1 (MQ=255)
ataACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATc < 1:2483433/62‑1 (MQ=255)
ataACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATc < 1:2445116/62‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:9716/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:513/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:2685755/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:2270027/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:2088057/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:2045111/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:1987663/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:898791/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:157639/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:173656/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:1784017/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:1819293/44‑1 (MQ=255)
aCCGGATCCCGGTGATTGCAGAAGGCGTAGAGAGTGAAGACGtt < 1:1901275/44‑1 (MQ=38)
tCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt < 1:1302196/38‑1 (MQ=255)
cccGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGa > 1:2744062/1‑61 (MQ=255)
cccGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGAt > 1:2321942/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGCCGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:1174878/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGCCGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:2481236/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTa > 1:660917/1‑37 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:3008647/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:304225/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:2770675/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:962529/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:1919295/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:1194270/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:2428895/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:2381594/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:2153916/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:2015715/1‑62 (MQ=255)
ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc > 1:745748/1‑62 (MQ=255)
aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:647389/62‑1 (MQ=255)
aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:464973/62‑1 (MQ=255)
aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:992518/62‑1 (MQ=255)
aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:3089739/62‑1 (MQ=255)
aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:3013224/62‑1 (MQ=255)
aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:2367505/62‑1 (MQ=255)
aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:1936723/62‑1 (MQ=255)
aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:1867837/62‑1 (MQ=255)
aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:1181957/62‑1 (MQ=255)
aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:1024827/62‑1 (MQ=255)
aGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:1503529/61‑1 (MQ=255)
aGGCGTAGAGAGGGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg < 1:1015200/61‑1 (MQ=255)
|
ATAACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAG > W3110S.gb/875595‑875680
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
