Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
977,292 |
+G |
coding (545/4461 nt) |
mukB → |
fused chromosome partitioning proteins |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 977,289 | 1 | . | G | 100.0%
| 30.5
/ NA
| 10 | L181R (CTG→CGG) | mukB | fused chromosome partitioning proteins |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (10/0); total (10/0) |
ACCACTCGCTGATGTTCGATCT‑GGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAC > W3110S.gb/977268‑977326
|
aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc > 1:1109382/1‑60 (MQ=255)
aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc > 1:1301731/1‑60 (MQ=255)
aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc > 1:1758010/1‑60 (MQ=255)
aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc > 1:2541628/1‑60 (MQ=255)
aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc > 1:2569239/1‑60 (MQ=255)
aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc > 1:2596627/1‑60 (MQ=255)
aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc > 1:2651137/1‑60 (MQ=255)
aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc > 1:2661857/1‑60 (MQ=255)
aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc > 1:944375/1‑60 (MQ=255)
aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc > 1:977985/1‑60 (MQ=255)
|
ACCACTCGCTGATGTTCGATCT‑GGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAC > W3110S.gb/977268‑977326
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
