Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,985,114 |
A→G |
G189G (GGT→GGC) |
araH ← |
fused L‑arabinose transporter subunits and membrane components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,985,114 | 0 | A | G | 100.0%
| 11.1
/ NA
| 5 | G189G (GGT→GGC) | araH | fused L‑arabinose transporter subunits and membrane components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/5); total (0/5) |
CAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG > W3110S.gb/1985075‑1985135
|
ctgggTATTACGACAAAAGGTGGTTTTATTCAGCAGCAAGCAAAACATGATCAGACAcgcg < 1:3053038/59‑1 (MQ=255)
cAGGGTATTACGACCAAAGGTGGTTTTATTCAGCAGCAAGCCAAAGATGATCAGACAcgcg < 1:3106215/61‑1 (MQ=255)
cAGGGTATTACGACCAAAGGTGGTTTTATTCAGCAGCAAGCCAAAGATGATCAGACAcgcg < 1:557257/61‑1 (MQ=255)
aGGGTATTACGACCAAAGGTGGTTTTATTCAGCAGCAAGCCAAAGATGATCAGACAcgcg < 1:295810/60‑1 (MQ=255)
aGGTGGTTTTATTCAGCAGCAAGCCAAAGATGATCAGACAcgcg < 1:2810685/44‑1 (MQ=38)
|
CAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG > W3110S.gb/1985075‑1985135
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
