Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,039,714 |
A→G |
G192G (GGT→GGC) |
yedV ← |
predicted sensory kinase in two‑component regulatory system with YedW |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,039,714 | 0 | A | G | 96.5%
| 93.8
/ ‑4.0
| 29 | G192G (GGT→GGC) | yedV | predicted sensory kinase in two‑component regulatory system with YedW |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (0/28); total (1/28) |
| Fisher's exact test for biased strand distribution p-value = 3.45e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 |
ATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTA > W3110S.gb/2039693‑2039754
|
ataATTCAGGGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:64105/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:2396021/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:979938/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:632287/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:595427/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:588344/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:567739/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:453477/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:3077332/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:3050954/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:3030203/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:2768683/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:2742228/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:2426059/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:229581/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:2172319/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:2157732/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:2129082/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:209369/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:2047088/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:181953/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:14598/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:1069322/62‑1 (MQ=255)
ataATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:1064569/62‑1 (MQ=255)
taATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:1062008/61‑1 (MQ=255)
taATTCAGCGCTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:1322576/61‑1 (MQ=255)
taATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGAtt > 1:1785702/1‑60 (MQ=255)
cgcTTCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:364938/53‑1 (MQ=255)
ttCCGTTACGCCACTCAACTTTTTGATCTCTCGCAACCCCGTCCTGATTa < 1:1576998/50‑1 (MQ=255)
|
ATAATTCAGCGCTTCCGTTACACCACTCAACTTTTTGATCTCTCGTAATCCCGTTCTGATTA > W3110S.gb/2039693‑2039754
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
