Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I2 R2	154	32.4	3140800	76.4%	2399571	54.5

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,464,537	T→C	V23V (GTA→GTG)	yfcX ←	fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,464,537	0	T	C	100.0%	41.7 / NA	13	V23V (GTA→GTG)	yfcX	fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (1/12); total (1/12)

AATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGT  >  W3110S.gb/2464480‑2464565

aatGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACg                           >  1:1781509/1‑61 (MQ=255)

                        cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:10661/62‑1 (MQ=255)

                        cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:1073063/62‑1 (MQ=255)

                        cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:1171720/62‑1 (MQ=255)

                        cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:1175428/62‑1 (MQ=255)

                        cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:1256032/62‑1 (MQ=255)

                        cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:2324631/62‑1 (MQ=255)

                        cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:2755977/62‑1 (MQ=255)

                        cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:362530/62‑1 (MQ=255)

                        cTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:745848/62‑1 (MQ=255)

                         tCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:1968274/61‑1 (MQ=255)

                         tCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:431254/61‑1 (MQ=255)

                         tCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt  <  1:676441/61‑1 (MQ=255)

AATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGT  >  W3110S.gb/2464480‑2464565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A