Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,595,882 |
C→T |
M131I (ATG→ATA) |
purC ← |
phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,595,882 | 0 | C | T | 100.0%
| 124.5
/ NA
| 38 | M131I (ATG→ATA) | purC | phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/9); total (29/9) |
TACGCGCCAGGTTCTCTTTGCTCACCCAGCCAAAGGTTTCGCAGTAAGATTCGTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCA > W3110S.gb/2595824‑2595937
|
tACGCGCCAGGTTCTCTTTGCTCACCCAGCCAAAGGTTTCGCAGTAAGATTCGTTGACTAt > 1:1537955/1‑61 (MQ=255)
ggTTCTCTTTGCTCACCCAGCCAAAGGTTTCGCAGTAAGATTCGTTGACTATCGGATCGTGc > 1:1088759/1‑62 (MQ=255)
tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAGCAGATCGaa < 1:2788859/62‑1 (MQ=255)
tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAGCAGATCGaa < 1:2792378/62‑1 (MQ=255)
tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:1055141/62‑1 (MQ=255)
tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:539915/62‑1 (MQ=255)
tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:675690/62‑1 (MQ=255)
tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:741238/62‑1 (MQ=255)
tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:1949564/62‑1 (MQ=255)
tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:1415334/62‑1 (MQ=255)
tCGCAGTAAGATTCGTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGaa < 1:3025584/62‑1 (MQ=255)
gTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTc > 1:564799/1‑61 (MQ=255)
gTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCa > 1:122568/1‑62 (MQ=255)
gTTGACTATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCa > 1:568616/1‑62 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:470035/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:498192/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:478395/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2860996/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:591544/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:743628/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:75607/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:797455/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:856558/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:907127/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:464056/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2939588/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2883227/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2760998/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2739015/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2711886/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2435849/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:2167179/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:1747964/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:1660392/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:1460608/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAgg > 1:1071571/1‑36 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTCGTTTTTCAg > 1:2367371/1‑35 (MQ=255)
ttGACTATCGGATCGTGCATGGCGTAGTTTTTCAgg > 1:109709/1‑36 (MQ=37)
|
TACGCGCCAGGTTCTCTTTGCTCACCCAGCCAAAGGTTTCGCAGTAAGATTCGTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCA > W3110S.gb/2595824‑2595937
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
