Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,845,313 |
T→C |
G252G (GGA→GGG) |
hycD ← |
hydrogenase 3, membrane subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,845,313 | 0 | T | C | 100.0%
| 11.9
/ NA
| 5 | G252G (GGA→GGG) | hycD | hydrogenase 3, membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (1/4); total (1/4) |
AGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCC > W3110S.gb/2845278‑2845344
|
aGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGCCCACCGGCGGTGaa < 1:1338117/50‑1 (MQ=255)
aGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGCCCACCGGCGGTGaa < 1:2945369/50‑1 (MQ=255)
aGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGCCCACCGGCGGTGaa < 1:3096580/50‑1 (MQ=255)
aCGATGGCAATCACCAGCGCCAGCAGCAGCCCACCGGCGGTGAAGGTTTCCATTTGTcccc < 1:542095/61‑1 (MQ=255)
cGATGGCAATCACCAGCGCCAGCAGCAGCCCACCGGCGGTGAAGGTTTcc > 1:706500/1‑50 (MQ=255)
|
AGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCC > W3110S.gb/2845278‑2845344
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
