Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,068,411 |
A→G |
V18A (GTA→GCA) |
mscS ← |
mechanosensitive channel |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,068,411 | 0 | A | G | 100.0%
| 103.8
/ NA
| 30 | V18A (GTA→GCA) | mscS | mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/9); total (21/9) |
TTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCT > W3110S.gb/3068388‑3068458
|
ttAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaaca > 1:3015361/1‑62 (MQ=255)
ttAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaac > 1:1222603/1‑61 (MQ=255)
ttAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaac > 1:3029064/1‑61 (MQ=255)
ttAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGaca > 1:1614539/1‑59 (MQ=255)
tAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaaca > 1:142844/1‑61 (MQ=255)
tAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaaca > 1:938/1‑61 (MQ=255)
tAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaaca > 1:722382/1‑61 (MQ=255)
tAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaaca > 1:669381/1‑61 (MQ=255)
tAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaaca > 1:291671/1‑61 (MQ=255)
tAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaaca > 1:287894/1‑61 (MQ=255)
tAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaaca > 1:2790349/1‑61 (MQ=255)
tAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaaca > 1:2746184/1‑61 (MQ=255)
tAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaaca > 1:2446965/1‑61 (MQ=255)
tAGCAGCAGCGCCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGacaaca > 1:2351178/1‑61 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt < 1:1992836/62‑1 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt > 1:960668/1‑62 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt < 1:1572286/62‑1 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt > 1:823278/1‑62 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt > 1:754712/1‑62 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt < 1:1662002/62‑1 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt > 1:183693/1‑62 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt < 1:617455/62‑1 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt > 1:593167/1‑62 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt < 1:2517216/62‑1 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt < 1:2019886/62‑1 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt < 1:2964/62‑1 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt > 1:2311334/1‑62 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt < 1:2347364/62‑1 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt > 1:2643873/1‑62 (MQ=255)
gcgcCTGGTTAGCTGCCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCt < 1:2598463/62‑1 (MQ=255)
|
TTAGCAGCAGCGCCTGGTTAGCTACCAGCCAGCTTCCCGCGCCGTTTATGCTATCGACAACATTCAAATCT > W3110S.gb/3068388‑3068458
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
