Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,551,647 |
G→A |
T263T (ACG→ACA) |
fdoG → |
formate dehydrogenase‑O, large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,551,647 | 0 | G | A | 92.3%
| 71.5
/ ‑1.1
| 26 | T263T (ACG→ACA) | fdoG | formate dehydrogenase‑O, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (24/0); total (24/2) |
| Fisher's exact test for biased strand distribution p-value = 3.08e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 |
AAAATTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACGCGTACGGCGGCGGTAGCTGACTACTATGCCCCTATTC > W3110S.gb/3551600‑3551684
|
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:2430782/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:995611/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:985805/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:800924/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:65958/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:343029/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:314111/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:29575/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:29145/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:2722585/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:2450539/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1073997/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:232437/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:212749/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:2121603/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1814735/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1623567/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1609282/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:157838/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:138046/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1358941/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1233678/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1116483/1‑57 (MQ=39)
aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagc > 1:1086437/1‑56 (MQ=39)
aaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACGCGTACGGCGGCGGTa < 1:3079755/62‑1 (MQ=255)
gATTGTGATCGATCCTCGCTTTACGCGTACGGCGGCGGTAGCTGACTACTATGCCCCTATTc < 1:2734746/62‑1 (MQ=255)
|
AAAATTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACGCGTACGGCGGCGGTAGCTGACTACTATGCCCCTATTC > W3110S.gb/3551600‑3551684
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
