Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,663,442 |
C→A |
A240S (GCG→TCG) |
rffG ← |
dTDP‑glucose 4,6‑dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,663,442 | 0 | C | A | 100.0%
| 10.7
/ NA
| 5 | A240S (GCG→TCG) | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/5); total (0/5) |
CACCAATATTATAGGTTTCACCGACTTTCCCGGTGGTCGCCACGCAATACAGCGCGCGGGCG > W3110S.gb/3663403‑3663464
|
caccaATATTATAGGTTTCACCGACTTTCCCGGTGGTCGACACGCAATACAGCGCGCGGGCg < 1:2220430/62‑1 (MQ=255)
caccaATATTATAGGTTTCACCGACTTTCCCGGTGGTCGACACGCAATACAGCGCGCGGGCg < 1:2593552/62‑1 (MQ=255)
caccaATATTATAGGTTTCACCGACTTTCCCGGTGGTCGACACGCAATACAGCGCGCGGGCg < 1:2943159/62‑1 (MQ=255)
accaATATTATAGGTTTCACCGACTTTCCCGGTGGTCGACACGCAATACAGCGCGCGGGCg < 1:1570072/61‑1 (MQ=255)
ccaATATTATAGGTTTCACCGACTTTCCCGGTGGTCGACACGCAATACAGCGCGCGGGCg < 1:418207/60‑1 (MQ=255)
|
CACCAATATTATAGGTTTCACCGACTTTCCCGGTGGTCGCCACGCAATACAGCGCGCGGGCG > W3110S.gb/3663403‑3663464
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
