Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,702,210 |
C→A |
S232S (TCG→TCT) |
rbsA ← |
fused D‑ribose transporter subunits and ATP‑binding components ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,702,210 | 0 | C | A | 100.0%
| 57.3
/ NA
| 19 | S232S (TCG→TCT) | rbsA | fused D‑ribose transporter subunits and ATP‑binding components ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (19/0); total (19/0) |
ATCTCAATCAGCGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGCCCATC > W3110S.gb/3702199‑3702260
|
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACtt > 1:1372477/1‑37 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGtccgt > 1:48402/1‑59 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGtccgt > 1:2270695/1‑59 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:830391/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:1088303/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:478932/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:3058716/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:2824951/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:274055/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:2719110/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:2435714/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:2348893/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:2073177/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:1598712/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:1592282/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:1523254/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:1335536/1‑62 (MQ=255)
atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:1157718/1‑62 (MQ=255)
atcGCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc > 1:1281722/1‑62 (MQ=255)
|
ATCTCAATCAGCGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGCCCATC > W3110S.gb/3702199‑3702260
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
