Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R2	90	19.5	1203533	67.3%	809977	57.3

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	569,274	A→G	Y414H (TAC→CAC)	hcp ←	hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	569,274	0	A	G	100.0%	99.5 / NA	29	Y414H (TAC→CAC)	hcp	hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (7/22); total (7/22)

GAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCC > minE/569221‑569304

gAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCt                        >  1:841752/1‑62 (MQ=255)

gAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCt                        >  1:698780/1‑62 (MQ=255)

gAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCt                        >  1:111990/1‑62 (MQ=255)

gAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCt                        >  1:1190787/1‑62 (MQ=255)

gAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCt                        >  1:606164/1‑62 (MQ=255)

gAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCt                        >  1:440233/1‑62 (MQ=255)

gAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCt                        >  1:1018571/1‑62 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:356104/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:919636/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:901644/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:714829/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:641256/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:584068/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:528978/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:505525/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:389572/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:315231/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:291534/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:220673/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:206818/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:1131678/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:1105002/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:1080317/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:1064512/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:1031880/60‑1 (MQ=255)

                        cGGCACGCTGGTGGCGAAATCGGTGAAGTGGGGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:572859/60‑1 (MQ=255)

                         ggCACGCTGGTGGCGAAATCGGTGAAGTGGTGGCGCGCGCCGCGTGCGCCGTCACAGcc  <  1:1022100/59‑1 (MQ=255)

                         ggCACGCTGGGGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:469243/59‑1 (MQ=39)

                               cTGGTGGCGAAATCGGTGAAGTGGTGGCGCTCGCCGCGTGCGCCGTCACAGcc  <  1:147683/53‑1 (MQ=255)

GAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCC > minE/569221‑569304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A