Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R2
|
90 |
19.5 |
1203533 |
67.3% |
809977 |
57.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,532,618 |
C→A |
L36L (CTG→CTT) |
aroC ← |
chorismate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,532,618 | 0 | C | A | 100.0%
| 104.8
/ NA
| 32 | L36L (CTG→CTT) | aroC | chorismate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/32); total (0/32) |
GCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGC > minE/1532602‑1532662
|
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:475562/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:991175/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:905181/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:896166/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:886431/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:87726/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:797365/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:768440/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:75919/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:674481/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:654024/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:618270/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:593809/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:56833/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:513086/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:478770/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:1032017/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:45513/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:445990/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:437897/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:371431/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:349014/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:239679/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:218791/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:1170278/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:1132220/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:1085642/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:108276/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:10438/61‑1 (MQ=255)
gCAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:1042165/61‑1 (MQ=255)
cAGGTCCGCTTCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:188748/60‑1 (MQ=255)
tCCGTAAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAgcgc < 1:463736/50‑1 (MQ=255)
|
GCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGC > minE/1532602‑1532662
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
