Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R2
|
90 |
19.5 |
1203533 |
67.3% |
809977 |
57.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,468,230 |
2 bp→GG |
coding (102‑103/864 nt) |
atpG → |
F1 sector of membrane‑bound ATP synthase, gamma subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,468,230 | 0 | A | G | 100.0%
| 48.8
/ NA
| 15 | K34K (AAA→AAG) | atpG | F1 sector of membrane‑bound ATP synthase, gamma subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/0); total (15/0) |
| * | minE | 2,468,231 | 0 | T | G | 100.0%
| 50.7
/ NA
| 15 | S35A (TCG→GCG) | atpG | F1 sector of membrane‑bound ATP synthase, gamma subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (15/0); total (15/0) |
AAGATCACTAAAGCGATGGAGATGGTCGCCGCTTCCAAAATGCGTAAATCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCA > minE/2468183‑2468292
||
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCa > 1:974987/1‑61 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCa > 1:722772/1‑61 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:470438/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:996161/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:861180/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:741164/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:701830/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:471405/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:415620/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:364516/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:1059549/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:222918/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:17261/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:1154262/1‑62 (MQ=255)
aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt > 1:12587/1‑62 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:605752/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:1056980/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:880484/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:115071/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:841818/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:841734/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:779440/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:757865/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:1173619/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:240583/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:587582/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:568281/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:555939/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:161749/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:1034121/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:37924/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:337927/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa < 1:279750/62‑1 (MQ=255)
tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGGCa < 1:384517/62‑1 (MQ=255)
||
AAGATCACTAAAGCGATGGAGATGGTCGCCGCTTCCAAAATGCGTAAATCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCA > minE/2468183‑2468292
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
