Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R2
|
90 |
19.5 |
1203533 |
67.3% |
809977 |
57.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,727,193 |
(A)5→6 |
intergenic (‑111/‑17) |
yheS ← / → kefG |
fused predicted transporter subunits and ATP‑binding components of ABC superfamily/component of potassium effux complex with KefB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,727,188 | 1 | . | A | 95.7%
| 149.4
/ 0.7
| 47 | intergenic (‑106/‑22) | yheS/kefG | fused predicted transporter subunits and ATP‑binding components of ABC superfamily/component of potassium effux complex with KefB |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (45/0); total (47/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
TGCCGCACCGGGCGCAATGGTAGCCCAAAACAGCGACTATACAC‑AAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTTGCTGC > minE/2727145‑2727240
|
tGCCGCACCGGGCGCAATGGTAGCCCAAAACAGCGACTATACAC‑AAAAA‑CCATACCGGGAgg > 1:777332/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGTGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:96805/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGatgatg > 1:655144/1‑37 (MQ=38)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGaaa > 1:1045273/1‑52 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:514662/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:992728/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:623006/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:626847/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:646733/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:651170/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:670923/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:689463/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:69894/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:785928/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:811019/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:81956/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:884906/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:892390/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:89995/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:90893/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:956935/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:972853/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:9919/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:1033087/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:298075/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:1072593/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:108863/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:1093910/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:1128137/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:1128926/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:1143287/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:1164584/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:1186014/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:172610/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:239348/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:268728/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:501040/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:299106/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:347180/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:37331/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:418103/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:422402/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:442199/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:449641/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:460875/1‑62 (MQ=255)
aCTATACACAAAAAA‑CCATACAGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:69125/1‑62 (MQ=255)
aCTATACAC‑AAAAACCCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTtgctgc > 1:315229/1‑62 (MQ=255)
|
TGCCGCACCGGGCGCAATGGTAGCCCAAAACAGCGACTATACAC‑AAAAA‑CCATACCGGGAGGGGGATGATGTCTCAGCCAGCGAAAGTTTTGCTGC > minE/2727145‑2727240
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
