Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F17 I0 R2
|
159 |
90.8 |
4763082 |
92.7% |
4415377 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,727,940 |
T→C |
5.7% |
F59F (TTT→TTC) |
kefB → |
potassium:proton antiporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,727,940 | 0 | T | C | 5.7%
| 262.2
/ 5.6
| 87 | F59F (TTT→TTC) | kefB | potassium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (32/50); new base C (2/3); total (34/53) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
GCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTTTGGC > minE/2727875‑2728007
|
gCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCgg < 1:2224816/70‑1 (MQ=255)
aGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAAc > 1:4440741/1‑71 (MQ=255)
gATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCg < 1:3511910/71‑1 (MQ=255)
ttGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGc < 1:532429/71‑1 (MQ=255)
ttGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGc < 1:2292576/71‑1 (MQ=255)
cAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTg < 1:1715241/71‑1 (MQ=255)
aaTTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGAAGAGATCCTCCACtttt > 1:4748749/1‑56 (MQ=255)
aTTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTgg > 1:2939699/1‑70 (MQ=255)
ggCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTAt > 1:4713408/1‑70 (MQ=255)
ggCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTAt > 1:3526484/1‑70 (MQ=255)
ggCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTAt > 1:2998394/1‑70 (MQ=255)
cGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCg < 1:3267309/57‑1 (MQ=255)
cGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCg < 1:2477674/57‑1 (MQ=255)
cGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCg < 1:420922/57‑1 (MQ=255)
cGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCt > 1:4493090/1‑70 (MQ=255)
cGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTg < 1:1099456/71‑1 (MQ=255)
gTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCt < 1:4695740/69‑1 (MQ=255)
ggggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATg < 1:2464811/70‑1 (MQ=255)
ggggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATg < 1:493993/70‑1 (MQ=255)
ggggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGt < 1:2425017/71‑1 (MQ=255)
gggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTg < 1:2656203/66‑1 (MQ=255)
ggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt < 1:4646396/71‑1 (MQ=255)
ggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt < 1:4209521/71‑1 (MQ=255)
ggCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt < 1:317286/71‑1 (MQ=255)
cTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGa > 1:2060657/1‑64 (MQ=255)
gggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatcat < 1:3729955/71‑1 (MQ=255)
gggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatcat > 1:3483788/1‑71 (MQ=255)
gggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatca < 1:3147406/70‑1 (MQ=255)
gggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc < 1:330661/69‑1 (MQ=255)
gggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc < 1:1992675/69‑1 (MQ=255)
ggTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGc < 1:1412224/47‑1 (MQ=255)
ggTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTc > 1:2162293/1‑44 (MQ=255)
gTTTATTAGCGACGTCGATGAGATCCTCCACTTTTc > 1:3913278/1‑36 (MQ=255)
tttATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCgg < 1:4257130/71‑1 (MQ=255)
tattaGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCg < 1:3296839/44‑1 (MQ=255)
tattaGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTgg < 1:964726/47‑1 (MQ=255)
tattaGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGt < 1:1087633/48‑1 (MQ=255)
attaGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGcc < 1:557554/70‑1 (MQ=255)
ttaGCGACGTCGATGAGATCCTCCCCTTTTCGGAACTCGGCGTGGTATTc > 1:1413526/1‑50 (MQ=255)
ttaGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt < 1:3621723/71‑1 (MQ=255)
taGCGACGTCGATGAGATCCTCCACTTTTCGGAAc < 1:1298597/35‑1 (MQ=255)
taGCGACGTCGATGAGATCCTCCACTTTTCGGAAc < 1:1750605/35‑1 (MQ=255)
taGCGACGTCGATGAGATCCTCCACTTTTCGGAAc < 1:3482154/35‑1 (MQ=255)
aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGGTGttt > 1:3363403/1‑57 (MQ=255)
aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt > 1:4633393/1‑57 (MQ=255)
aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt > 1:4226880/1‑57 (MQ=255)
aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt > 1:2364593/1‑69 (MQ=255)
aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt > 1:4210206/1‑69 (MQ=255)
aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt > 1:4379704/1‑69 (MQ=255)
aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTg < 1:387358/70‑1 (MQ=255)
aGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTg < 1:1930888/70‑1 (MQ=255)
gACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatcatc > 1:2539653/1‑60 (MQ=255)
gACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGtt < 1:4621741/71‑1 (MQ=255)
aCGTCGATGAGATCCTCCACTTTTCGGAACTCGGCg < 1:3586715/36‑1 (MQ=255)
aCGTCGATGAGATCCTCCACTTTTCGGAACTCGGCg < 1:1881973/36‑1 (MQ=255)
aCGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTg < 1:1075409/71‑1 (MQ=255)
cGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGa < 1:3429086/71‑1 (MQ=255)
cGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGACTTGAGTTGa < 1:849008/71‑1 (MQ=255)
gTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt < 1:532668/51‑1 (MQ=255)
gTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGttt < 1:1190390/51‑1 (MQ=255)
gTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGtt < 1:786480/50‑1 (MQ=255)
tCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCt < 1:4213584/43‑1 (MQ=255)
tCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTg < 1:2565740/63‑1 (MQ=255)
tCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTg < 1:1374368/63‑1 (MQ=255)
gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTa > 1:3127969/1‑36 (MQ=255)
gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGa > 1:4252973/1‑43 (MQ=255)
gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGa > 1:4154181/1‑43 (MQ=255)
gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGa > 1:704844/1‑43 (MQ=255)
gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGa > 1:2999009/1‑43 (MQ=255)
gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGaa > 1:4703463/1‑68 (MQ=255)
gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGaa > 1:4618819/1‑68 (MQ=255)
gATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATcc > 1:3647375/1‑71 (MQ=255)
tGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCt < 1:300833/39‑1 (MQ=255)
aTCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGc < 1:3817706/51‑1 (MQ=255)
aTCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGc < 1:4343118/51‑1 (MQ=255)
tcctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCaaa < 1:1922085/71‑1 (MQ=255)
cctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc > 1:4174389/1‑43 (MQ=255)
cctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCt > 1:4294094/1‑51 (MQ=255)
cctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCt > 1:2574112/1‑51 (MQ=255)
cctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAAc > 1:2568751/1‑71 (MQ=255)
cctccACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGaa > 1:2982663/1‑60 (MQ=255)
ctccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAAc < 1:2078591/70‑1 (MQ=255)
tccACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACtt > 1:3124170/1‑71 (MQ=255)
cACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc > 1:501783/1‑39 (MQ=255)
cACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc > 1:643000/1‑39 (MQ=255)
cTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTatc < 1:483864/37‑1 (MQ=255)
cTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt < 1:2916384/46‑1 (MQ=255)
cTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCtt < 1:3809123/46‑1 (MQ=255)
ttttCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTTTGGc > 1:4533831/1‑70 (MQ=255)
tttCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGa > 1:3556734/1‑46 (MQ=255)
tttCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGa > 1:3356565/1‑46 (MQ=255)
tttCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGa > 1:3409014/1‑46 (MQ=255)
|
GCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTTTGGC > minE/2727875‑2728007
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
