Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F17 I0 R2 159 90.8 4763082 92.7% 4415377 60.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,728,325 C→T 29.0% L188L (CTG→TTG)  kefB → potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,728,3250CT29.0% 196.5 / 101.1 124L188L (CTG→TTG) kefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (49/39);  new base T (15/21);  total (64/60)
Fisher's exact test for biased strand distribution p-value = 1.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 129 total aligned reads displayed.

GGCGTTAGTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTATTGCAGCTTC  >  minE/2728255‑2728395
                                                                      |                                                                      
ggCGTTAGTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGc                                                                        >  1:4004862/1‑71 (MQ=255)
 gCGTTAGTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCt                                                                       <  1:3038630/71‑1 (MQ=255)
   gTTAGTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTgg                                                                     <  1:1248576/71‑1 (MQ=255)
    ttAGTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTgg                                                                     >  1:1958692/1‑70 (MQ=255)
    ttAGTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTgg                                                                     >  1:2032013/1‑70 (MQ=255)
       gTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGt                                                                  <  1:117866/70‑1 (MQ=255)
       gTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGt                                                                  <  1:2196777/70‑1 (MQ=255)
       gTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGt                                                                  <  1:2756529/70‑1 (MQ=255)
       gTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGt                                                                  <  1:481275/70‑1 (MQ=255)
       gTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGt                                                                  <  1:1668670/70‑1 (MQ=255)
        tGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGttt                                                                <  1:4320486/71‑1 (MQ=255)
        tGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGttt                                                                <  1:912259/71‑1 (MQ=255)
        tGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGttt                                                                <  1:3427001/71‑1 (MQ=255)
        tGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGttt                                                                <  1:1488257/71‑1 (MQ=255)
          ccGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGt                                                              <  1:4092419/71‑1 (MQ=255)
          ccGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGt                                                              <  1:2085322/71‑1 (MQ=255)
            gttgttGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCg                                                            <  1:704608/71‑1 (MQ=255)
             ttgttgGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCg                                                            <  1:3761481/70‑1 (MQ=255)
             ttgttgGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCg                                                            <  1:3291432/70‑1 (MQ=255)
               gttgGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCa                                                         >  1:2334209/1‑71 (MQ=255)
                ttgGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCa                                                         <  1:1034112/70‑1 (MQ=255)
                  ggCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTg                                                               >  1:1674177/1‑62 (MQ=255)
                  ggCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGt                                                              >  1:3491603/1‑63 (MQ=255)
                   gCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCt                                                     <  1:3618055/71‑1 (MQ=255)
                   gCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCt                                                     <  1:3116637/71‑1 (MQ=255)
                      gggTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCATGCTGAt                                                  <  1:4548515/71‑1 (MQ=255)
                      gggTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCATGCTGAt                                                  <  1:3655077/71‑1 (MQ=255)
                       ggTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGAtt                                                 >  1:4126502/1‑71 (MQ=255)
                         tCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCgg                                                           >  1:4291567/1‑59 (MQ=255)
                           ggCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCt                                                     >  1:4267438/1‑63 (MQ=255)
                           ggCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATtggtg                                             >  1:4756657/1‑71 (MQ=255)
                               gACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCgg                                                           <  1:3109168/53‑1 (MQ=255)
                               gACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCAt                                                        <  1:904460/56‑1 (MQ=255)
                               gACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCAt                                                        <  1:1284580/56‑1 (MQ=255)
                               gACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGgc                                          >  1:4482991/1‑70 (MQ=255)
                                aCGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATtgg                                               <  1:405755/64‑1 (MQ=255)
                                aCGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATtgg                                               <  1:3453481/64‑1 (MQ=255)
                                aCGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATtgg                                               <  1:3016653/64‑1 (MQ=255)
                                 cGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCt                                       <  1:2754107/71‑1 (MQ=255)
                                   aaCATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGAtt                                                 <  1:2642564/59‑1 (MQ=255)
                                    aCATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTAtt                                    >  1:930486/1‑71 (MQ=255)
                                    aCATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTAtt                                    >  1:1505892/1‑71 (MQ=255)
                                    aCATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTAtt                                    <  1:2619226/71‑1 (MQ=255)
                                    aCATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTGTGTCGGCATGCTGATTGGTGGGCGCTAtt                                    <  1:1760077/71‑1 (MQ=255)
                                     cATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTAttt                                   <  1:4223074/71‑1 (MQ=255)
                                     cATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTAttt                                   <  1:3818698/71‑1 (MQ=255)
                                     cATTTCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTAttt                                   <  1:2255593/71‑1 (MQ=255)
                                     cATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCg                                                                   <  1:2458220/39‑1 (MQ=255)
                                     cATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTAtt                                    >  1:23475/1‑70 (MQ=255)
                                     cATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTAtt                                    >  1:2102934/1‑70 (MQ=255)
                                     cATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTAGCGTTTGTCGGCATGCTGATTGGTGGGCGCTAttt                                   <  1:4419212/71‑1 (MQ=255)
                                     cATTTCGACTGGATGAAGATCGGCATGAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTAttt                                   <  1:1052619/71‑1 (MQ=255)
                                       tttCGACTGGATGAAGGTCGGCATGAAGGTGTTGGCGt                                                                  <  1:4193282/38‑1 (MQ=255)
                                       tttCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTAc                                 >  1:2249488/1‑71 (MQ=255)
                                       tttCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTAc                                 >  1:4174726/1‑71 (MQ=255)
                                        ttCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACt                                >  1:472485/1‑71 (MQ=255)
                                         tCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGc                                                      >  1:2226782/1‑48 (MQ=255)
                                         tCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGc                                                      >  1:3673333/1‑48 (MQ=255)
                                         tCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTa                                  >  1:3203874/1‑68 (MQ=255)
                                         tCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACt                                >  1:4054077/1‑70 (MQ=255)
                                          cGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTAc                                 >  1:3017051/1‑68 (MQ=255)
                                           gACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCgg                                                           <  1:44990/41‑1 (MQ=255)
                                           gACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCgg                                                           <  1:2834015/41‑1 (MQ=255)
                                           gACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCgg                                                           <  1:677891/41‑1 (MQ=255)
                                           gACTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCgg                                                           <  1:1048344/41‑1 (MQ=255)
                                             cTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCa                                                         >  1:46886/1‑41 (MQ=255)
                                             cTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCa                                                         >  1:1343123/1‑41 (MQ=255)
                                             cTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCa                                                         >  1:1434140/1‑41 (MQ=255)
                                             cTGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCa                                                         >  1:137352/1‑41 (MQ=255)
                                             cTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCa                                                         >  1:2152464/1‑41 (MQ=255)
                                             cTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCa                                                         >  1:4611934/1‑41 (MQ=255)
                                             cTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCa                                                         >  1:754987/1‑41 (MQ=255)
                                             cTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTc                           >  1:650915/1‑71 (MQ=255)
                                              tGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCa                                                         >  1:3773264/1‑40 (MQ=255)
                                              tGGATGAAGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCa                                                         >  1:3778617/1‑40 (MQ=255)
                                              tGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGgcgc                                        <  1:2444831/57‑1 (MQ=255)
                                                gATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTcc                          <  1:1355655/69‑1 (MQ=255)
                                                 aTGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCgg                        >  1:3647121/1‑70 (MQ=255)
                                                  tGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTa                      >  1:3090361/1‑71 (MQ=255)
                                                    aaGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTAtt                    <  1:1249359/71‑1 (MQ=255)
                                                    aaGGTCGGCATGAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTAt                     >  1:3636501/1‑70 (MQ=255)
                                                         cGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATtg                                                >  1:1577828/1‑38 (MQ=255)
                                                         cGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCt               >  1:1025582/1‑71 (MQ=255)
                                                          ggCATGAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCt               <  1:2147162/70‑1 (MQ=255)
                                                          ggCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATtgg                                               <  1:1777690/38‑1 (MQ=255)
                                                             aTGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTAt           >  1:1336354/1‑71 (MQ=255)
                                                             aTGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTAt           >  1:1674590/1‑71 (MQ=255)
                                                              tGAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGc                <  1:924302/65‑1 (MQ=255)
                                                              tGAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGc                <  1:1302970/65‑1 (MQ=255)
                                                               gAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTAttt                                   <  1:746480/45‑1 (MQ=255)
                                                               gAAGGTGTTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTATTg         >  1:1522754/1‑71 (MQ=255)
                                                               gAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTATTg         >  1:2331670/1‑71 (MQ=255)
                                                                aaGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGTGTCCGGTa                      >  1:4342631/1‑57 (MQ=255)
                                                                aaGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTATTg         >  1:4694314/1‑70 (MQ=255)
                                                                aaGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTATTg         <  1:1879216/70‑1 (MQ=255)
                                                                   gTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTc                   >  1:3927987/1‑57 (MQ=255)
                                                                   gTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTATTGCAGc     >  1:63916/1‑71 (MQ=255)
                                                                     gCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTa                                  >  1:3927581/1‑40 (MQ=255)
                                                                     gCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTATTGCAGCtt   >  1:2037417/1‑71 (MQ=255)
                                                                      cTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCgg                        <  1:3649803/49‑1 (MQ=255)
                                                                      |                                                                      
GGCGTTAGTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTATTGCAGCTTC  >  minE/2728255‑2728395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A