Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R2
|
90 |
19.5 |
1203533 |
67.3% |
809977 |
57.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,882,260 |
+GT |
coding (106/381 nt) |
ytfH → |
predicted transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,882,256 | 1 | . | G | 100.0%
| 119.9
/ NA
| 34 | G34? (GGG→GGN) | ytfH | predicted transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (34/0); total (34/0) |
| * | minE | 2,882,256 | 2 | . | T | 100.0%
| 108.2
/ NA
| 34 | G34G (GGG→GGT) | ytfH | predicted transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (34/0); total (34/0) |
CCAGCCGTTGGGGG‑‑GTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGA > minE/2882243‑2882303
||
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTAcgc > 1:50523/1‑40 (MQ=25)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCa > 1:789174/1‑51 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCAt > 1:756495/1‑52 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCg > 1:1016349/1‑54 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGc > 1:854456/1‑61 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGc > 1:268860/1‑61 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:679297/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:443672/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:476534/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:485712/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:48770/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:557527/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:439925/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:791010/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:832021/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:848832/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:873397/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:888324/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:4302/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:393362/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:34387/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:285919/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:256193/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:221251/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:220267/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:136648/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:120679/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:1194475/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:1112224/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:1077686/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:1077195/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:1056870/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCg > 1:1018567/1‑62 (MQ=255)
ccAGCCGTTGGGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGC‑TTAGCGa > 1:599578/1‑62 (MQ=255)
||
CCAGCCGTTGGGGG‑‑GTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGA > minE/2882243‑2882303
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
