Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R2
|
64 |
60.0 |
3197630 |
92.7% |
2964203 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,714,239 |
C→A |
R41L (CGC→CTC) |
nirB ← |
nitrite reductase, large subunit, NAD(P)H‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,714,239 | 0 | C | A | 100.0%
| 126.8
/ NA
| 38 | R41L (CGC→CTC) | nirB | nitrite reductase, large subunit, NAD(P)H‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (10/28); total (10/28) |
CCAGCGACAGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAG > minE/2714171‑2714294
|
ccAGCGACAGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgagc < 1:196536/71‑1 (MQ=255)
aCAGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGttct < 1:2770433/71‑1 (MQ=255)
aGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGttctt > 1:90391/1‑70 (MQ=255)
ttCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGtt < 1:1544082/62‑1 (MQ=255)
cggcggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGaa > 1:2826058/1‑70 (MQ=255)
ggcggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGaaaa < 1:2712868/71‑1 (MQ=255)
cggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGCTCATAAGCCATGAGCCGTTCTTCACAGAAAACg > 1:2371737/1‑71 (MQ=255)
gtgtGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgagc < 1:1019373/51‑1 (MQ=255)
gtgtGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgagc < 1:553342/51‑1 (MQ=255)
gtgtGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgagc < 1:2333182/51‑1 (MQ=255)
gtgtGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgagc < 1:1255845/51‑1 (MQ=255)
gtgtGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACgg > 1:905096/1‑70 (MQ=255)
gtgtGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGt < 1:112007/71‑1 (MQ=255)
tgtgGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTa < 1:97359/71‑1 (MQ=255)
gtgAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAata < 1:2861409/70‑1 (MQ=255)
gtgAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAata < 1:764034/70‑1 (MQ=255)
gagaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCa > 1:1600775/1‑69 (MQ=255)
gaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGt < 1:2800770/71‑1 (MQ=255)
gaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGT‑ATATCAAAGtt < 1:1434007/71‑1 (MQ=255)
aaGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGtt < 1:2901114/71‑1 (MQ=255)
aaGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGtt < 1:1208407/71‑1 (MQ=255)
aaGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGt < 1:141588/70‑1 (MQ=255)
aGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgagc < 1:1656764/38‑1 (MQ=255)
aGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTg < 1:488243/71‑1 (MQ=255)
gTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTgg < 1:2373443/71‑1 (MQ=255)
tAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGaaaa < 1:647060/52‑1 (MQ=255)
tAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGaaaa < 1:3069442/52‑1 (MQ=255)
tAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGaaaa < 1:2840315/52‑1 (MQ=255)
tAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGaaaa < 1:2886508/52‑1 (MQ=255)
tAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGc < 1:619664/71‑1 (MQ=255)
tAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGc < 1:1794643/71‑1 (MQ=255)
aaGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCa > 1:3181377/1‑62 (MQ=255)
aaGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCa > 1:1127251/1‑62 (MQ=255)
cGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCa < 1:1029774/70‑1 (MQ=255)
tACGCTGTCATAAGCGATGATCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTa < 1:1035143/70‑1 (MQ=255)
tACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCa < 1:1952236/49‑1 (MQ=255)
tACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCa < 1:192921/49‑1 (MQ=255)
tACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCa < 1:890600/49‑1 (MQ=255)
tACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCa < 1:1378122/49‑1 (MQ=255)
cgcgGTCATAAGCGATGAGCGGTTCTTCACAGAAAACg > 1:2461711/1‑38 (MQ=255)
cgcgGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCa < 1:2701743/47‑1 (MQ=255)
gcgGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATc > 1:2098310/1‑69 (MQ=255)
aTAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCaag > 1:1832242/1‑66 (MQ=255)
cGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGt < 1:965736/39‑1 (MQ=255)
|
CCAGCGACAGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAG > minE/2714171‑2714294
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
