Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R2
|
251 |
90.0 |
5200563 |
86.9% |
4519289 |
59.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,614,466 |
G→A |
16.7% |
intergenic (‑91/+126) |
eutH ← / ← eutG |
predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,614,466 | 0 | G | A | 16.7%
| 119.9
/ 14.8
| 48 | intergenic (‑91/+126) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base G (17/23); new base A (3/5); total (20/28) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 |
TTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTA > minE/1614398‑1614530
|
ttatttatTGGCGGATGCGTCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAc < 1:3870269/71‑1 (MQ=255)
tatttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAc < 1:4947492/70‑1 (MQ=255)
gcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCAt > 1:413232/1‑71 (MQ=39)
cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:1548697/1‑71 (MQ=39)
cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:4821866/1‑71 (MQ=39)
cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:503915/1‑71 (MQ=39)
cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:5023755/1‑71 (MQ=39)
gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:1030584/71‑1 (MQ=39)
gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:495362/71‑1 (MQ=39)
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:270339/1‑71 (MQ=38)
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:3753546/1‑71 (MQ=38)
gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:638910/71‑1 (MQ=255)
gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:4344863/71‑1 (MQ=255)
gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:400448/71‑1 (MQ=255)
gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:3494483/71‑1 (MQ=255)
cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGc < 1:645112/69‑1 (MQ=11)
cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGc < 1:284218/69‑1 (MQ=11)
cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc < 1:1768806/71‑1 (MQ=255)
cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc > 1:677866/1‑71 (MQ=255)
cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc < 1:3801480/71‑1 (MQ=255)
cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc < 1:3337926/71‑1 (MQ=255)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:4874531/46‑1 (MQ=38)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:4770734/46‑1 (MQ=38)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:1305743/46‑1 (MQ=38)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:3583129/46‑1 (MQ=38)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:1698984/46‑1 (MQ=38)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:2677118/46‑1 (MQ=38)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:2228795/46‑1 (MQ=38)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:24955/46‑1 (MQ=38)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:2256118/46‑1 (MQ=38)
aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:1454422/1‑71 (MQ=255)
aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcg > 1:4521302/1‑70 (MQ=255)
tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:3216677/1‑46 (MQ=38)
tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2876117/1‑46 (MQ=38)
tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2626482/1‑46 (MQ=38)
tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2363660/1‑46 (MQ=38)
tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2332232/1‑46 (MQ=38)
accGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCaa > 1:5169620/2‑45 (MQ=38)
cGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCgcgc < 1:1875160/70‑1 (MQ=255)
aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc > 1:3971104/1‑71 (MQ=255)
cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg > 1:3481713/1‑71 (MQ=14)
cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg > 1:2517284/1‑71 (MQ=14)
cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg > 1:711761/1‑71 (MQ=14)
cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc < 1:2539557/70‑1 (MQ=255)
aGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAAc < 1:322710/70‑1 (MQ=255)
ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa < 1:4770001/71‑1 (MQ=18)
ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa < 1:2912512/71‑1 (MQ=18)
ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa < 1:863468/71‑1 (MQ=18)
|
TTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTA > minE/1614398‑1614530
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
