Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
488,810 |
(A)5→4 |
coding (3051/3363 nt) |
kefA → |
fused mechanosensitive channel proteins |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 488,806 | 0 | A | . | 100.0%
| 193.2
/ NA
| 45 | coding (3047/3363 nt) | kefA | fused mechanosensitive channel proteins |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (45/0); total (45/0) |
CTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCA > W3110S.gb/488749‑488860
|
ctgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaaagtg < 1:1881274/64‑4 (MQ=255)
ctgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaaagtg < 1:1224359/64‑4 (MQ=255)
ctgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaaagtg < 1:634222/64‑4 (MQ=255)
ctgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaaagtg < 1:1284038/64‑4 (MQ=255)
ctgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaaagtg < 1:2088384/64‑4 (MQ=255)
ctgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaaagtg < 1:2490148/64‑4 (MQ=255)
ctgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaaagtg < 1:1528417/64‑4 (MQ=255)
ctgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaaagtg < 1:78075/64‑4 (MQ=255)
ctgacACTACTACGCGTCTGGTGATCCGTCTCGGCGGGGCCTATGGCTCCGATCTGGaaaagtg < 1:2076697/64‑4 (MQ=255)
tgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaaagtg < 1:996231/63‑4 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAg > 1:715228/1‑35 (MQ=38)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAccc > 1:1307130/1‑52 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTg > 1:1898928/1‑59 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2495749/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:283422/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2483065/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2477227/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2130472/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:288061/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:229197/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:442191/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:506090/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:537050/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:640262/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:657773/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:724969/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:77463/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:806002/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:865070/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:966129/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:989439/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1765471/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1010196/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1245239/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1254542/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1299930/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1305548/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1458239/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1538795/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1571401/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1571631/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1632120/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1759001/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2254154/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1864021/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1945092/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:196514/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2129009/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2129275/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1006980/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2171938/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2216805/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2245283/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAACGCGGCGACTGAGCACCCAAGGGTGATGc > 1:2309287/1‑63 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTCAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1946135/1‑64 (MQ=255)
|
CTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCA > W3110S.gb/488749‑488860
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
