Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
488,810 |
(A)5→4 |
coding (3051/3363 nt) |
kefA → |
fused mechanosensitive channel proteins |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 488,806 | 0 | A | . | 100.0%
| 207.0
/ NA
| 48 | coding (3047/3363 nt) | kefA | fused mechanosensitive channel proteins |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (39/9); total (39/9) |
CTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGA > W3110S.gb/488749‑488863
|
ctgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaaagtg < 1:2176232/64‑4 (MQ=255)
cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTa > 1:1101875/1‑64 (MQ=255)
cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTa > 1:2805255/1‑64 (MQ=255)
cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTa > 1:1811799/1‑64 (MQ=255)
cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTa > 1:2910077/1‑64 (MQ=255)
cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTa > 1:2925115/1‑64 (MQ=255)
cgcgTCTGGTGATCCGTATCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTa > 1:1422632/1‑64 (MQ=255)
tCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGaa > 1:2085638/1‑65 (MQ=255)
tCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGaa > 1:2995853/1‑65 (MQ=255)
tCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGaa > 1:477236/1‑65 (MQ=255)
tCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGa > 1:1663064/1‑65 (MQ=255)
tCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGa > 1:600724/1‑65 (MQ=255)
tCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGa > 1:846996/1‑65 (MQ=255)
tCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGa > 1:2016666/1‑65 (MQ=255)
tgcccTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCaa < 1:1007591/62‑1 (MQ=255)
tGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCaa < 1:3103158/65‑1 (MQ=255)
tGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCaa < 1:787590/65‑1 (MQ=255)
tGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCaa < 1:2776014/65‑1 (MQ=255)
tGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCaa < 1:233455/65‑1 (MQ=255)
tGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCaa < 1:937019/65‑1 (MQ=255)
tGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCaa < 1:1974950/65‑1 (MQ=255)
tGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCaa < 1:1215348/65‑1 (MQ=255)
tGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCaa < 1:1338817/65‑1 (MQ=255)
tacGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2257823/3‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGc > 1:1853486/1‑63 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGc > 1:318145/1‑63 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1623610/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1126228/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1152926/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:523121/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:47833/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:1338015/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:405034/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:3105838/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:211066/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2936396/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2799956/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2682854/1‑64 (MQ=255)
tCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa > 1:2177868/1‑64 (MQ=255)
cGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGa > 1:2939642/1‑65 (MQ=255)
cGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGa > 1:1665316/1‑65 (MQ=255)
cGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGa > 1:1709591/1‑65 (MQ=255)
cGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGa > 1:446637/1‑65 (MQ=255)
cGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGa > 1:2809418/1‑65 (MQ=255)
cGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGa > 1:185161/1‑65 (MQ=255)
cGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGa > 1:519701/1‑65 (MQ=255)
cGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGa > 1:1314563/1‑65 (MQ=255)
cGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGa > 1:831473/1‑65 (MQ=255)
cGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGa > 1:2026888/1‑65 (MQ=255)
|
CTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGA > W3110S.gb/488749‑488863
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
