minE Evolution: LB_Evolution
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Metadata |
Mutations |
Converged Mutations |
Fixed Mutations |
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Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A1 F28 I2 R1 | 330 | 0.0 | 2544031 | 91.9% | 2337964 | 62.1 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,473,254 | Δ1 bp | pseudogene (6149/8622 nt)|pseudogene (851/3324 nt) | ydbA → ydbA → |
ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,473,253 | 0 | G | . | 100.0% | 20.1 / NA | 6 | pseudogene (6148/8622 nt) pseudogene (850/3324 nt) | ydbA ydbA | ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (0/6); total (0/6) | |||||||||||
ATACCAGCGGTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCG > W3110S.gb/1473245‑1473310 | aTACCAGC‑GTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCg < 1:1917093/65‑1 (MQ=255)aTACCAGC‑GTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCg < 1:2102148/65‑1 (MQ=255)aTACCAGC‑GTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCg < 1:347952/65‑1 (MQ=255)aTACCAGC‑GTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCg < 1:383562/65‑1 (MQ=255)aTACCAGC‑GTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCg < 1:842151/65‑1 (MQ=255) tACCAGC‑GTGGCATCATCAATAACTTAGTATAACTTGACAAGGGTGCCGTCATTAAAAATGCCg < 1:1495149/64‑1 (MQ=255) | ATACCAGCGGTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCG > W3110S.gb/1473245‑1473310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |