Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,799,263 |
G→A |
S132S (AGC→AGT) |
pheT ← |
phenylalanine tRNA synthetase, beta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,799,263 | 0 | G | A | 100.0%
| 14.3
/ NA
| 7 | S132S (AGC→AGT) | pheT | phenylalanine tRNA synthetase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (7/0); total (7/0) |
GATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATG > W3110S.gb/1799218‑1799281
|
gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAAt > 1:1973607/1‑63 (MQ=255)
gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAAt > 1:2218376/1‑63 (MQ=255)
gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATg > 1:1035735/1‑64 (MQ=255)
gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATg > 1:1140598/1‑64 (MQ=255)
gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATg > 1:162231/1‑64 (MQ=255)
gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATg > 1:2453375/1‑64 (MQ=255)
gATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATg > 1:2487835/1‑64 (MQ=255)
|
GATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATG > W3110S.gb/1799218‑1799281
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
