Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,298,568 |
T→C |
E49E (GAA→GAG) |
ccmE ← |
periplasmic heme chaperone |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,298,568 | 0 | T | C | 100.0%
| 152.4
/ NA
| 41 | E49E (GAA→GAG) | ccmE | periplasmic heme chaperone |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/18); total (23/18) |
CAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGAGCGCAG > W3110S.gb/2298528‑2298627
|
cAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTcc > 1:801189/1‑65 (MQ=255)
cAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTcc > 1:1135543/1‑65 (MQ=255)
cAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTcc > 1:1397860/1‑65 (MQ=255)
cAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTc > 1:1669022/1‑64 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:2055806/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:988701/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:788617/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:510087/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:48859/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:2144377/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1036576/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1887728/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1878121/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1593227/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1037957/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1142582/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1463435/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1493605/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1659020/65‑1 (MQ=255)
cGACTTCCGGCATTTGCCGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1690366/65‑1 (MQ=255)
gACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:686660/64‑1 (MQ=255)
ccGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:312990/59‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:447977/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:97801/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:958130/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:861861/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:1077441/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:1096015/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:647876/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:1400972/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:464093/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:1572322/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:317604/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:309616/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:218948/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:2185441/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:2154637/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:1723643/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:209468/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:1814922/1‑64 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag > 1:1531913/1‑64 (MQ=255)
|
CAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGAGCGCAG > W3110S.gb/2298528‑2298627
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
