Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,298,568 |
T→C |
E49E (GAA→GAG) |
ccmE ← |
periplasmic heme chaperone |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,298,568 | 0 | T | C | 100.0%
| 248.0
/ NA
| 67 | E49E (GAA→GAG) | ccmE | periplasmic heme chaperone |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/48); total (19/48) |
TCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGAGCGCAG > W3110S.gb/2298522‑2298627
|
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:2722619/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:2444896/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:1918909/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:2471291/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:1837711/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:2041350/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:1825923/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:2574241/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:1536603/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:2171900/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:2736850/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:3015017/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:3105688/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:364408/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:226559/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:440379/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAgaga > 1:729554/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCAATTGCTGAGTCTCACGCTTGCCGTAgaga > 1:2296740/1‑65 (MQ=255)
tCCCGCCAACGCGCAGACGCAGACCGACTTCCGGCATTTGCTGAGTCTCACGCTTg > 1:246844/1‑56 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGTTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:773839/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:2286230/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:2314744/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:2375466/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1039771/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:2514717/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:2651474/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:2720282/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:3019469/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:3107112/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:389723/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:681137/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:2247837/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1289575/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1143484/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1490407/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1832801/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:1900848/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:2162807/65‑1 (MQ=255)
cGACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAgaga < 1:2246869/65‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:78869/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:802103/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:1056186/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:567725/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:552388/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:42749/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:413546/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:1128302/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:337409/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:1204118/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:1302575/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:1860953/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:1499048/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:1771860/41‑1 (MQ=255)
tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:3089163/41‑1 (MQ=255)
gCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt < 1:1319306/40‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:1109374/64‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:965107/64‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:2034980/64‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:1014178/64‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:2537029/64‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:1086075/64‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:182222/64‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:2681593/64‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:1423970/64‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:2795752/64‑1 (MQ=255)
gAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:2272597/64‑1 (MQ=255)
aGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcag < 1:2641987/63‑1 (MQ=255)
|
TCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGAGCGCAG > W3110S.gb/2298522‑2298627
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
