Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,383,756 |
+C |
coding (174/1671 nt) |
menD ← |
bifunctional 2‑oxoglutarate decarboxylase and SHCHC synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,383,752 | 1 | . | C | 100.0%
| 36.4
/ NA
| 11 | H60D (CAT→GAT) | menD | bifunctional 2‑oxoglutarate decarboxylase and SHCHC synthase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (11/0); total (11/0) |
CCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATG‑CCCCAACCCACGCTCATCGAA > W3110S.gb/2383710‑2383773
|
ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGc > 1:2375358/1‑57 (MQ=39)
ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa > 1:1269689/1‑65 (MQ=255)
ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa > 1:1431654/1‑65 (MQ=255)
ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa > 1:1964502/1‑65 (MQ=255)
ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa > 1:20438/1‑65 (MQ=255)
ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa > 1:2096408/1‑65 (MQ=255)
ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa > 1:2187671/1‑65 (MQ=255)
ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa > 1:2241286/1‑65 (MQ=255)
ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa > 1:2377051/1‑65 (MQ=255)
ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa > 1:860280/1‑65 (MQ=255)
ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa > 1:861739/1‑65 (MQ=255)
|
CCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATG‑CCCCAACCCACGCTCATCGAA > W3110S.gb/2383710‑2383773
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
