Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,559,548 |
T→C |
I293T (ATT→ACT) |
hemF → |
coproporphyrinogen III oxidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,559,548 | 0 | T | C | 100.0%
| 97.1
/ NA
| 27 | I293T (ATT→ACT) | hemF | coproporphyrinogen III oxidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/27); total (0/27) |
GGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGG > W3110S.gb/2559491‑2559564
|
gggAATATTATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:2383895/65‑1 (MQ=255)
gggAATATGATTATCAGCCATAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:2072981/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:878171/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:1005314/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:668262/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:48622/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:450639/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:383483/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:2390332/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:2380188/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:2367970/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:2319640/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:2249538/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:2078958/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:1602614/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:1472542/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:1347287/65‑1 (MQ=255)
gggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:1317096/65‑1 (MQ=255)
aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:819667/56‑1 (MQ=255)
aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg < 1:2010930/65‑1 (MQ=255)
aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg < 1:2456640/65‑1 (MQ=255)
aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg < 1:450405/65‑1 (MQ=255)
aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg < 1:619885/65‑1 (MQ=255)
aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg < 1:627407/65‑1 (MQ=255)
aTTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg < 1:844031/65‑1 (MQ=255)
aTTATCAGCCAAAAGATGGCAGACCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg < 1:703363/65‑1 (MQ=255)
ggCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTc < 1:1347595/39‑1 (MQ=255)
|
GGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGG > W3110S.gb/2559491‑2559564
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
