Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,649,364 |
Δ1 bp |
coding (1580/4962 nt) |
yfhM ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,649,361 | 0 | A | . | 100.0%
| 118.2
/ NA
| 29 | coding (1583/4962 nt) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (0/29); total (0/29) |
AATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTA > W3110S.gb/2649313‑2649381
|
aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCaaa < 1:1180248/65‑1 (MQ=255)
aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCaaa < 1:1851662/65‑1 (MQ=255)
aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCaaa < 1:524063/65‑1 (MQ=255)
aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCaaa < 1:1533608/65‑1 (MQ=255)
aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCaaa < 1:526955/65‑1 (MQ=255)
tCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa < 1:2181512/65‑1 (MQ=255)
tCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa < 1:849925/65‑1 (MQ=255)
tCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa < 1:1336223/65‑1 (MQ=255)
cAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa < 1:2039533/64‑1 (MQ=255)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAATTTGCCCTTGc < 1:767106/44‑1 (MQ=38)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:757415/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:243419/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:2431016/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:2326116/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:2293726/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:921011/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:1909083/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:2029235/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:1947695/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:1004108/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:1896633/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:1806801/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:1774524/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:1694466/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:1668725/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:1524950/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:1387570/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc < 1:1020755/44‑1 (MQ=39)
gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGGTGCCCTTGc < 1:1142368/44‑1 (MQ=38)
|
AATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTA > W3110S.gb/2649313‑2649381
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
