Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,732,756 |
T→A |
D25V (GAC→GTC) |
clpB ← |
protein disaggregation chaperone |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,732,756 | 0 | T | A | 100.0%
| 14.2
/ NA
| 6 | D25V (GAC→GTC) | clpB | protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (6/0); total (6/0) |
TCATTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCATCG > W3110S.gb/2732724‑2732785
|
tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:1964225/1‑62 (MQ=255)
tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:2228566/1‑62 (MQ=255)
tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:2228910/1‑62 (MQ=255)
tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:388895/1‑62 (MQ=255)
tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:527095/1‑62 (MQ=255)
tCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCg > 1:812888/1‑62 (MQ=255)
|
TCATTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCATCG > W3110S.gb/2732724‑2732785
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
