Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I3 R1
|
178 |
99.0 |
1887089 |
95.3% |
1798395 |
63.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,800,301 |
A→C |
F119C (TTC→TGC) |
pheS ← |
phenylalanine tRNA synthetase, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,800,301 | 0 | A | C | 100.0%
| 165.9
/ NA
| 44 | F119C (TTC→TGC) | pheS | phenylalanine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (25/19); total (25/19) |
CTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAG > W3110S.gb/1800254‑1800341
|
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:1061615/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:506577/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:521550/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:420685/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:57770/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:275788/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:197202/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:689824/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:1818130/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:787709/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:149665/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:1088696/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:1351462/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:1280871/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:815945/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:124021/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:1010316/65‑1 (MQ=255)
cTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:833398/65‑1 (MQ=255)
ttCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCg < 1:648722/64‑1 (MQ=255)
aCGGTAAAGCCAAGCTTACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:1855596/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCa > 1:1235857/1‑64 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:918848/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:557230/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:562831/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:655735/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:688165/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:741367/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:789490/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:79593/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:466998/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:45435/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:436573/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:387147/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:362630/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:296154/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:1860266/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:1848549/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:1769309/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:1737945/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:1721897/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:1583452/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:12531/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:1155778/1‑65 (MQ=255)
aCGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg > 1:1118410/1‑65 (MQ=255)
|
CTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAG > W3110S.gb/1800254‑1800341
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
