Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I3 R1
|
178 |
99.0 |
1887089 |
95.3% |
1798395 |
63.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,611,108 |
T→C |
F185L (TTC→CTC) |
hyfR → |
DNA‑binding transcriptional activator, formate sensing |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,611,108 | 0 | T | C | 100.0%
| 764.4
/ NA
| 200 | F185L (TTC→CTC) | hyfR | DNA‑binding transcriptional activator, formate sensing |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/200); total (0/200) |
Only 100 of 200 total aligned reads displayed.
TCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAA > W3110S.gb/2611062‑2611154
|
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1648361/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1879733/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1873398/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1867660/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1862895/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1843048/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1715578/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1670511/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1655360/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:284693/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1642228/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1630991/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:159472/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1570972/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1549284/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1536804/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1527144/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1014464/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:307172/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:339183/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:387211/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:46246/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:477647/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:484720/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:548098/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:564424/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:697188/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:743000/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:819429/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:930704/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:942730/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:987326/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:991606/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1253693/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1524353/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1429480/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1118430/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1076945/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1296821/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1062107/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:141890/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1126188/62‑1 (MQ=255)
tctcGACGATCTGATCGCTGACGGCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:760297/62‑1 (MQ=255)
ctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:796903/61‑1 (MQ=255)
ctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1044325/61‑1 (MQ=255)
gaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:573195/55‑1 (MQ=255)
aTCTGATGGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1744057/54‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:706910/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1129726/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:406314/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:511240/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:529814/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1177029/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1183936/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:561910/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1111814/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:586470/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:601041/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:362027/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:77693/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:806042/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:896307/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:965837/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1036630/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1004534/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:316904/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1336180/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1282392/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1391056/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1426017/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1263026/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1228940/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1881736/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1591196/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:217117/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1447318/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:122691/53‑1 (MQ=255)
cGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:332779/53‑1 (MQ=255)
gCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:252081/52‑1 (MQ=255)
gACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcat < 1:1268982/43‑2 (MQ=39)
gACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa < 1:1378112/49‑1 (MQ=255)
cGCTCGGGAGATCCATCATTTTCTCGGTCTGGCTtcag < 1:1050155/38‑1 (MQ=37)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:927322/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:1601258/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:88549/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:867177/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:1470892/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:166101/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:750055/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:1788632/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:108713/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:656970/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:631658/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:1801853/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:1834326/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:1274288/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:1884137/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:1134284/65‑1 (MQ=255)
cGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:1208792/65‑1 (MQ=255)
atTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa < 1:1611932/52‑1 (MQ=255)
|
TCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAA > W3110S.gb/2611062‑2611154
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
