Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I3 R1	178	99.0	1887089	95.3%	1798395	63.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,093,680	G→A	H19H (CAC→CAT)	yggR ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,093,680	0	G	A	100.0%	236.9 / NA	65	H19H (CAC→CAT)	yggR	predicted transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (64/1); total (64/1)

ATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACA > W3110S.gb/3093636‑3093719

aTTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTAt                      <  1:1513711/64‑1 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTTCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1429175/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGCCGTTATGCTTTACACTAAGGGCcaca  >  1:1479068/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:204751/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:319069/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:29116/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:254822/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:212519/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:206136/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1732180/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:197963/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:181030/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1802479/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1788294/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1785739/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1776881/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1746548/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1671498/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:336379/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:361586/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:474777/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:519216/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:534190/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:559194/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:733471/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:774137/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:779783/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:795074/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:818438/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:868025/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:871311/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:880109/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:970740/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:982680/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1426249/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1067288/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1098345/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1170924/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1216727/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:123397/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1234269/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1240890/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1266314/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1272094/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1328485/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1330527/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1332101/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1362021/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1370308/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1386608/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1701715/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1440732/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1489640/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1498139/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1523276/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1532280/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:153308/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1546534/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:157177/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1574131/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1578555/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1583593/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1660048/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:1016477/1‑65 (MQ=255)

                   aTCGTGCGGGCCAGGCGCAGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca  >  1:246995/1‑65 (MQ=255)

ATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACA > W3110S.gb/3093636‑3093719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A