Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I3 R1	178	99.0	1887089	95.3%	1798395	63.2

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,411,012	+A	coding (878/966 nt)	dusB →	tRNA‑dihydrouridine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,411,009	1	.	A	81.8%	26.6 / 2.1	11	coding (875/966 nt)	dusB	tRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (0/9); total (2/9)
Fisher's exact test for biased strand distribution p-value = 1.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCC‑AAATGACCAGTTTCGGCGCACATTCAACGCCAT  >  W3110S.gb/3410960‑3411042

tACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGACCACGCTCC‑AAATGACCAGTTTCg                    >  1:766237/1‑65 (MQ=255)

tACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCC‑AAATGCCCAGTTTCg                    >  1:1014999/1‑65 (MQ=255)

                   acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:1065523/65‑1 (MQ=255)

                   acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:10914/65‑1 (MQ=255)

                   acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:1398566/65‑1 (MQ=255)

                   acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:1412252/65‑1 (MQ=255)

                   acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:146183/65‑1 (MQ=255)

                   acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:1491750/65‑1 (MQ=255)

                   acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:237714/65‑1 (MQ=255)

                   acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:520488/65‑1 (MQ=255)

                   acGTTTCCTGGTATCTCCAGGAACACGCTCCAAAATGACCAGTTTCGGCGCACATTCAACGCCAt  <  1:916259/65‑1 (MQ=255)

TACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCC‑AAATGACCAGTTTCGGCGCACATTCAACGCCAT  >  W3110S.gb/3410960‑3411042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A