Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
928,130 |
C→T |
V496V (GTG→GTA) |
cydC ← |
fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 928,130 | 0 | C | T | 97.8%
| 135.7
/ ‑4.0
| 45 | V496V (GTG→GTA) | cydC | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (0/44); total (0/45) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 |
AGGATCTGGCTTTCGGTTGTGGCATCTAAGCCTTCGGTAGGTTCATCCAGCAACACCAGTGGCGCATCATGTAACAGCGCACGGGCGATAG > W3110S.gb/928077‑928167
|
aGGATCTGACTTTCGGTTGTGTCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1752395/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:349065/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2634427/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2662693/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2701719/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2710101/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2767977/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2793932/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2799432/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2937107/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:3028100/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:3032576/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:330845/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2509206/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:403028/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:440410/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:453102/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:510934/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:532278/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:586414/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:808568/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:88847/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:956168/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1821858/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1085991/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1185581/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1405922/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1581645/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1591943/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1672259/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:176699/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1774567/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:187026/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1927053/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1945640/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2092394/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2152158/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2227160/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:244681/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2479649/64‑1 (MQ=255)
aGGATCTGACTTTCGGTTGTGGCATCTAACCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2467802/64‑1 (MQ=255)
aGGATCTGACATTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:1135274/64‑1 (MQ=255)
ggATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTATGTTCATCCAGCAATACCAgcggcg < 1:1549933/63‑1 (MQ=255)
ggATCTGACTTTCGGTTGTGGCATCTAAGCCTTCTGTAGGTTCATCCAGCAATACCAgcggcg < 1:2957300/63‑1 (MQ=255)
ccTTCGGTAGGTTCATCCAGCAACACCAGTGGCGCATCATGTAACAGCGCACGGGCGATAg < 1:1082478/61‑1 (MQ=255)
|
AGGATCTGGCTTTCGGTTGTGGCATCTAAGCCTTCGGTAGGTTCATCCAGCAACACCAGTGGCGCATCATGTAACAGCGCACGGGCGATAG > W3110S.gb/928077‑928167
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
