Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,281,208 |
A→G |
L525L (CTA→CTG) |
yejF → |
fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,281,208 | 0 | A | G | 100.0%
| 186.7
/ NA
| 50 | L525L (CTA→CTG) | yejF | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (33/17); total (33/17) |
GAGTATACGCGTCAGCTACTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGC > W3110S.gb/2281191‑2281265
|
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:589435/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:2557975/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:2392869/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:2303023/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:448432/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:723993/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:1988546/65‑1 (MQ=255)
cgcgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCaa < 1:683025/64‑1 (MQ=255)
cgcgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCaa < 1:2683628/64‑1 (MQ=255)
cgcgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCaa < 1:2703730/64‑1 (MQ=255)
cgcgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCaa < 1:2725996/64‑1 (MQ=255)
cgcgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCaa < 1:19191/64‑1 (MQ=255)
cgcgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCaa < 1:1748586/64‑1 (MQ=255)
cgcgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCaa < 1:3070931/64‑1 (MQ=255)
cgcgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCaa < 1:1316991/64‑1 (MQ=255)
cgcgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCaa < 1:644239/64‑1 (MQ=255)
cgcgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTACTTTGAAAAGGGTTCGGCaa < 1:1144821/64‑1 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGCCGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:1328690/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCaaat > 1:45764/1‑62 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:926528/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:696394/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:2774141/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:561736/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:2709322/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:2684447/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:782852/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:251630/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:2318283/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:2249295/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:929978/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:1970117/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:1662641/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:1416041/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:1415906/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:94098/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:1230912/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:1070338/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAt > 1:520489/1‑63 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:976228/1‑64 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:677491/1‑64 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:613688/1‑64 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:313157/1‑64 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:3044973/1‑64 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:3044105/1‑64 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:2938873/1‑64 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:2575114/1‑64 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:2548651/1‑64 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:2061179/1‑64 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:1384788/1‑64 (MQ=255)
tCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGc > 1:1119021/1‑64 (MQ=255)
|
GAGTATACGCGTCAGCTACTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATTGC > W3110S.gb/2281191‑2281265
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
